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Then, the goal proteins or subdomains of them containing the target lysine may be produced recombinantly and the methylation also examined at protein degree. The horizontal axis represents the H3 sequence and the vertical axis represents the amino acids by which the corresponding row is mutated. A) Discrimination factors of NSD1 for the recognition of amino acid residues on the positions subsequent to the goal lysine (K36 within the experiment shown here).
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